4 edition of Mammalian Cell Transformation: Mechanisms of Carcinogenesis and Assays for Carcinogens (Carcinogenesis : a Comprehensive Survey Vol 9) found in the catalog.
Mammalian Cell Transformation: Mechanisms of Carcinogenesis and Assays for Carcinogens (Carcinogenesis : a Comprehensive Survey Vol 9)
J. Carl Barrett
by Lippincott Williams & Wilkins
Written in English
|Contributions||Raymond W. Tennant (Editor)|
|The Physical Object|
|Number of Pages||480|
Carcinogens or their metabolites are reactive electrophiles, which react with sites in DNA. Mammalian cell culture in vivo test systems (Drosophila, fish, rodents) Cell culture transformation assays Assays for DNA binding or damage, chromosome damage. IARC Classification Scheme of Human Carcinogenic Risk. Carcinogens that are non-genotoxic, non-apoptotic, and non-cytotoxic to the cell may still contribute to carcinogenesis in an epigenetic manner, by directly affecting gene expression during transcription, translation, and post-translational events.
Toxicity and Dose Determination.- Cell Treatment.- The Metabolic Activation System.- Staining and Scoring of Slides.- Evaluation Criteria.- Data Bases.- Overall Assessment.- References.- Section D: Transformation Assays.- 19 In Vitro Transformation Assays Using Mouse Embryo Cell Lines: Balb/c-3T3 Cells.- In Vitro Transformation of Balb/c-3T3. been recognized to be carcinogens in humans, raising the risk for breast and uterine cancer. As part of the search for the mechanism of hormone-induced carcinogenesis, various types of DNA damage have been detected which have been induced by oestrogens in cell-free systems, in cells in culture, or in vivo.
This book reviews the conduct and use of long- and short-term assays for the detection of chemical carcinogens, taking into account the progress made in the understanding of the process of carcinogenesis and the scientific basis of the various endpoints used in the assays. implicated in cell transformation. Rather, a role for estrogen metabolism seems to be important in estrogen-induced cell transfor-mation or carcinogenesis. No reports on the cell transforming activity and carcinogenic-ity of other estrogen blockers, e.g., EM and ICI ,, are available. Mechanisms of Cell Transformation by Estrogens.
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Based on the Symposium on Cell Transformation Assays: Application to Studies of Mechanisms of Carcinogenesis and to Carcinogen Testing held on Septemberat Research Triangle Park, N.C.; sponsored by the National Toxicology Program, National Institute of.
Mammalian cell transformation: mechanisms of carcinogenesis and assays for carcinogens / editors, J. Carl Barrett, Raymond W. Tennant Raven Press New York Wikipedia Citation Please see Wikipedia's template documentation for further citation fields that may be required. Cell Transformation Assays.
The most widely used of cell transformation assay include the Syrian hamster embryo (SHE) assay, the low-pH SHE assay, the Balb/c 3T3 assay, and the C3H10T1/2 assay [–], which rely on changes in mammalian cell colony morphology and focus formation following exposure to chemical substances .
The transformation of mammalian cells in vitro has been used to study the mechanism of carcinogenesis. There are many cell transformation assays, each having a different end-point such as changes in morphology, plating efficiency, serum requirement, nuclear size, enzyme activity, growth in semi-solid agar, cyto-skeletal structure and by: 6.
A variety of mammalian cell culture systems exist in which morphologic transformation is a reliable indicator of carcinogenic potential of the test agent (DiPaolo and Casto, ; Heidelberger, ).
These systems represent the closest short-term in vitro correlation to animal models for the study of carcinogenic by: 2. Abstract. Immortalization is a prerequisite for the clonal evolution and malignant transformation of normal mammalian cells in culture. In order to gain a mechanistic insight into the genetics of carcinogen-induced cellular immortality, a cell culture assay has been developed based on the use of freshly explanted Syrian hamster dermal (SHD) fibroblasts.
in vitromorphological transformation of mammalian cells in culture some 35 years mal cells to carcinogens, or by expressing els of carcinogenesis. Cell transformation assays. These chemicals can include promoters, which accelerate the multistage process of carcinogenesis. Some cell culture assays, such as the cell transformation assay, may be useful in detecting promoting activity (Abernethy et al., ; Frazelle et al., ), but there is still a need for short-term tests for promotion (IARC, ; NTP, pertinent to transformation assay procedures and to try to im prove the assay protocol so that cell transformation could be used reliably for screening environmental carcinogens.
The dis cussions of the Working Group made it possible to formulate recommendations for the procedures of cell transformation as says.
Author(s): Barrett,J Carl(James Carl); Tennant,Raymond W; National Toxicology Program (U.S.); Symposium on Cell Transformation Assays: Application to Studies of Mechanisms of Carcinogenesis and to Carcinogen Testing,( Research Triangle Park, N.C.) Title(s): Mammalian cell transformation: mechanisms of carcinogenesis and assays for carcinogens/ editors, J.
Carl. With the progress in molecular and cell biology, studies with cell model systems have produced many important conceptual advances for our understanding of the mechanism of carcinogenesis.
In parallel, animal models are required to provide the tissue and the systemic contexts of carcinogenesis and to validate molecular predictions and targeting. It was concluded that the combination of Salmonella and SAs for the DNA-reactive carcinogens and in vitro cell transformation assays for non-genotoxic carcinogens permits the identification of a.
The 19th annual meeting of the European Environmental Mutagen Society was held in Rhodes, Greece, from October 21st to 26th, The programme was chosen to explore what is currently known about the mechanisms of mutagenesis and carcinogenesis, induced by.
As background to the studies of chemical and radiation carcinogenesis, the book surveys knowledge of cell transformation and carcinogen metabolism.
Among the topics reviewed are the transforming genes involved in human malignancy, the genetics and epigenetics of neoplasia, and the single-hit and multi-hit concepts of hepatocarcinogenesis.
In vitro cell transformation assays have been proposed for predicting the carcinogenic potential of chemicals. Morphological transformation can be induced by exposing mammalian cells to carcinogens.
The cellular and molecular processes involved in cell transformation in the cultures are assumed to be similar to those of in vivo carcinogenesis. (A) In the standard protocol of the BALB/c-3T3-cell transformation assay cells are treated with MCA as tumor initiator (day 1–4) and TPA as tumor promotor (day 8–21).
After 42 days cells were. Molecular mechanism of in vitro transformation of mammalian cells by chemical carcinogens. di Mayorca G, Mishra N, Kimball P.
Bibl Haematol, (40), 01 Jan Cited by: 0. model systems. Most of our in vitro transformation studies have been performed in C3H lOT'/z cells, an in vitro transformation assay system originally developed by Reznikoff et al.
(4, 5). In this cell line, carcinogen treatment of cells leads to the formation of transformed foci within a background monolayer of normal-appearing cells, as. Stages of chemical carcinogenesis: • Basic mechanism of chemical carcinogenesis is by induction of mutation in the proto-oncogenes and antioncogenes.
• The phenomena of cellular transformation by chemical carcinogens (as also other carcinogens) is a progressive process involving 3 sequential stages: initiation, promotion and progression In Vivo Assay to Screen for Mutagens/Carcinogens in the Nematode C.
Elegans Pages Lew, K. (et al.) In Vitro Carcinogenesis with Cell Lines. Pages Heidelberger, Charles. Preview Buy Chap95 € Modulation of Induced Quantitative Mammalian Cell Transformation.
Pages DiPaolo, Joseph A. Mammalian cell transformation: mechanisms of carcinogenesis and assays for carcinogens. ed. by J.C. Barrett, R.W.
Tennantv. The role of chemicals and radiation in the etiologyof cancer. ed. by E. Huberman, S.H. Barr --v. I: The Fundamental Biology.- Section A: Mutagenesis and Other Short-Term Tests.- 1 An Overview of Short-Term Testing.- 2 Genetic Toxicology: Applications and Testing Strategies.- Applications and Testing Strategies.- References.- 3 Consequences of Genotoxic Effects.- Categories of Genotoxic Alterations.- Gene Mutation.- Definition.- Effects of Gene Mutation.- Mechanisms of Mutation .to induce cell transformation is consistent with whole animal carcinogenicity studies (1,2,4,5,).
This indicates that results for induction ofmorphological transformation in 1OT1/2 cells with nickel compounds correlates with the results of whole animal carcinogenesis assays using nickel compounds.